yvqK [2019-08-09 11:44:49]

ATP:cob(I)alamin adenosyltransferase

locus: BSU_33150

pI: 5.21

mw: 21.34 kDa

protein length: 193 aa

gene length: 582 bp

function: unknown

product: ATP:cob(I)alamin adenosyltransferase

essential: no

ec: 2.5.1.17

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.13|Biosynthesis/ acquisition of cobalamine (B12))]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,400,537 3,401,118

The protein

Catalyzed reaction/ biological activity

• ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide (according to Swiss-Prot)

Protein family

• Cob(I)alamin adenosyltransferase family (single member, according to UniProt)

Structure

• [PDB|1RTY]

Expression and Regulation

Operons

• genes: [gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]-[gene|1DC4A3D7E93677B0EAD15F43C80E50FD98527EBC|yvrB]-[gene|128EBAE9C14C7BB75173DF912853A6FCE86ABCE4|yvrA]-[gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]

  regulatory mechanism

  • [regulon|B12 riboswitch|B12 riboswitch]: termination, upon binding of B12 [Pubmed|14704351], in [regulon|B12 riboswitch|B12 riboswitch]

  regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) ([SW|B12 riboswitch]) [Pubmed|14704351]

Biological materials

Mutant

• MGNA-B042 (yvqK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1041 NBRP B. subtilis, Japan]
• BKE33150 ([gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCTGAATCTCCTTTA, downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC
• BKK33150 ([gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCTGAATCTCCTTTA, downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC

References

• 17588214,14704351